Scientist I, Protein Purification & Expression

Overview

Nurix Therapeutics is a clinical stage biopharmaceutical company focused on the discovery, development, and commercialization of targeted protein degradation medicines, the next frontier in innovative drug design aimed at improving treatment options for patients with cancer and inflammatory diseases. Powered by a fully AI-integrated discovery engine capable of tackling any protein class, and coupled with unparalleled ligase expertise, Nurix’s dedicated team has built a formidable advantage in translating the science of targeted protein degradation into clinical advancements. Nurix aims to establish degrader-based treatments at the forefront of patient care, writing medicine’s next chapter with a new script to outmatch disease.

Position

Nurix Therapeutics, Inc. is seeking a detail-oriented and experienced Protein Expression Scientist to join the DEL and Protein Sciences team. This individual will work cross functionally with internal stakeholders to plan, coordinate, and execute all operational activities required for analytical scale and preparative scale expression of recombinant proteins in baculovirus and mammalian cell systems. Additionally, they will be responsible for documentation of expression conditions and results in internal databases and electronic lab notebook systems.

Responsibilities:

• Highly proficient in maintaining insect cell and mammalian cell cultures.
• Excellent knowledge and high degree of familiarity with all steps in baculovirus expression of recombinant proteins, including clone design, generation and purification of bacmids, raising and titering of baculovirus, conducting MOI studies, cell culture of Sf9 and/or Tni/Hi5 cells, and harvesting of biomass on the scale of 1 L – 20 L.
• Highly proficient in maintaining insect cell and mammalian cell cultures.
• Strong working knowledge of protein characterization steps, including SDS-PAGE analysis, size exclusion chromatography, and/or thermal stability (e.g. nanoDSF).

Requirements

• Ph.D. or equivalent with 2+ years of relevant experience
• This position requires 2+ years of experience working with insect cell lines and baculovirus expression of recombinant proteins. Additional experience working with recombinant protein expression in mammalian cells (e.g. HEK293) is a plus. Industry experience preferred with exemplified ability to overexpress milligram amounts of protein for structural and functional studies for multiple protein classes.
• Able to work in dynamic, fast-paced environment with experience prioritizing work across multiple projects.
• Detail-oriented with strong record keeping skills.
• Past experience purifying recombinant proteins (e.g. affinity chromatography, ion exchange, or size exclusion chromatography) is a bonus.
• Must be able to lift 50+ pounds comfortably.
• Onsite Position

Salary Range:

125K - 151K plus bonus and equity

Fit with Nurix Culture and Values

• Strong team orientation; highly collaborative
• Solutions and results-oriented focus
• Hands-on approach; resourceful and open to diverse points of view